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KMID : 0811720100140030185
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Korean Journal of Physiology & Pharmacology
2010 Volume.14 No. 3 p.185 ~ p.189
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Activation of PPAR? Attenuates IFN? and IL-1?-induced Cell Proliferation in Astrocytes: Involvement of IL-6 Independent Pathway
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Lee Jin-Koo
Seo Eun-Min
Lee Sang-Soo
Park Soo-Hyun
Sim Yun-Beom
Jung Jun-Sub
Kim Seon-Mi
Suh Hong-Won
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Abstract
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The present study demonstrates the effect of fibrates, agonists of PPAR? on cytokines-induced proliferation in primary cultured astrocytes. Alone or combination treatment with cytokines, such as IL-1? (10 ng/ml), IFN? (10 ng/ml), and TNF-? (10 ng/ml) cause a significant increase of cell proliferation in a time-dependent manner. Treatment of astrocytes with bezafibrate and fenofibrate (0, 5, and 10 ?M) reduced the IFN? and IL-1?-induced cell proliferation in a dose-dependent manner. To address the involvement of IL-6 on the IFN? and IL-1?-induced cell proliferation, released IL-6 level was measured. IFN? and IL-1? cause an increase of released IL-6 protein level in a time-dependent manner. Furthermore, pretreatment with IL-6 antibody (0, 0.1, 1, 2.5, and 5 ng/ml) dose-dependently inhibited the IFN? and IL-1?-induced cell proliferation. However, bezafibrate and fenofibrate did not affect increased mRNA and protein levels of IL-6 in IFN? and IL-1?-stimulated astrocytes. Taken together, these results clearly suggest that activation of PPAR? attenuates the IFN? and IL-1?-induced cell proliferation through IL-6 independent pathway.
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KEYWORD
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Astrocytes, Bezafibrate, Fenofibrate, IL-6, Proliferation
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